Rapid Screening of Pathogens in Water

Description

Protozoan parasites such as Cryptosporidium parvum and Giardia duodenalis are recognized etiologic agents of waterborne disease outbreaks. A common factor with these microorganisms is the lack of simple and rapid methods for their detection and identification in water. Because current methods for detection of microorganisms in water are based on lengthy conventional culturing or microscopic enumeration techniques, this project developed a simple and rapid screening assay for selected microbial pathogens by adapting molecular methods for use in a convenient 96-well microplate format. which offers high throughput, rapid reading and potential for automation at low cost. List of Tables
List of Figures
Foreword
Acknowledgments
Executive Summary
Chapter 1: Introduction
Chapter 2: Materials and Methods
Chapter 3: Results and Discussion
Chapter 4: Conclusions and Recommendations
Appendix A: Standard Operating Procedure for Microplate Hybridization – Approach PCM/ML-DUTP
Appendix B: Standard Operating Procedure for Microplate
Hybridization – Approach ACM/SL-PRB
References
List of Abbreviations

Additional information